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利用 SSR 標記鑑定香菇單核體及雜交後代

微生物學通報 Feb. 20, 2016, 43(2): 444?455 Microbiology China

//ri.ope2.info/doc/baaaefe302020740bf1e9b6e.html/wswxtbcn

tongbao@//ri.ope2.info/doc/baaaefe302020740bf1e9b6e.html

DOI: 10.13344/j.microbiol.china.150335

Foundation item: Modern Agriculture Industry Technical System, Ministry of Agriculture of China (No. CARS-24);

The Seed Special Program, Shanghai Municipal Agriculture Commission (No. 6-2012); Shanghai Scientific and Technological Talents Program (No. 13XD1424700)

*Corresponding author: Tel: 86-21-62209760; E-mail: s6*******@//ri.ope2.info/doc/baaaefe302020740bf1e9b6e.html

Received: April 20, 2015; Accepted: July 17, 2015; Published online (//ri.ope2.info/doc/baaaefe302020740bf1e9b6e.html ): September 14, 2015

基金項目:現代農業產業技術體系建設專項資金項目(No. CARS-24);上海市農委種業專項項目(No. 滬農科種字

(2012)第6號);上海市科技人才計劃項目(No. 13XD1424700)

*通訊作者:Tel :86-21-62209760;E-mail :s6*******@//ri.ope2.info/doc/baaaefe302020740bf1e9b6e.html

收稿日期:2015-04-20;接受日期:2015-07-17;優先數字出版日期(//ri.ope2.info/doc/baaaefe302020740bf1e9b6e.html):2015-09-14

生物實驗室

利用SSR 標記鑑定香菇單核體及雜交後代

巫萍1,2 章爐軍2 張丹2 尚曉冬2 譚琦2 宋春豔2*

(1. 南京農業大學生命科學學院 江蘇 南京 210095)

(2. 上海市農業科學院食用菌研究所 農業部南方食用菌資源利用重點實驗室 國家食用菌工程技術研究中心

國家食用菌加工技術研發分中心 上海市農業遺傳育種重點開放實驗室 上海 201403)

摘 要:【新世代集運電話】應用SSR 標記可加快香菇單核體的製備進程,並提高鑑定單核體及相關雜交菌株的準確性,促進香菇遺傳育種研究。

關鍵詞:簡單重複序列,香菇,原生質體單核體,隨機擴增多態性DNA ,孢子單核體,雜交子鑑定

Identification of Xianggu (Lentinula edodes ) monokaryons and hybrid

progenies using SSR markers

WU Ping 1,2 ZHANG Lu-Jun 2 ZHANG Dan 2 SHANG Xiao-Dong 2 TAN Qi 2

SONG Chun-Yan 2*

(1. College of Life Sciences, Nanjing Agriculture University, Nanjing, Jiangsu 210095, China)

(2. Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, P . R. China; National Engineering Research Center of Edible Fungi, National R&D Center for Edible Fungi Processing, Key Laboratory of Agriculture Genetics and Breeding of Shanghai, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences,

Shanghai 201403, China)